Lesi sisho siphakamisa umbuzo: "Hhayi-ke, hlobo luni lwesitsha se-cryogenic lesi uma singakwazi ukusetshenziswa ku-nitrogen ewuketshezi?"
Akupheli iviki lapho singabuzwa khona ukuthi sichaze lo mshwana wokuzihlangula obonakala uxakile ovela kuwo wonke amakhasi omkhiqizo we-cryovial ngaphandle kokunaka umkhiqizi, kungakhathaliseki ukuthi ivolumu ingakanani futhi noma ngabe intambo yangaphakathi i-cryovial noma intambo yangaphandle e-cryovial.
Impendulo ithi: Lena indaba yecala hhayi umbuzo mayelana nekhwalithi ye-cryovial.
Ake sichaze.
Njengamashubhu amaningi ahlala isikhathi eside elabhorethri, ama-cryovials enziwa nge-polypropylene eqinile.
Ubukhulu be-polypropylene bunquma ububanzi bokushisa obuphephile.
Amashubhu amaningi ayi-conical angu-15mL no-50mL anezindonga ezincane ezibeka umkhawulo ekusebenziseni kwawo ukusebenza emazingeni okushisa angekho ngaphansi kuka -86 kuya ku-90 Celsius.
Izindonga ezizacile ziphinde zichaze ukuthi kungani amashubhu angu-15mL kanye no-50mL angalulekwa ukuthi ajikelezwe ngamanani ngokushesha kuno-15,000xg njengoba ipulasitiki ijwayele ukuqhekeka futhi iqhekeke uma isetshenziswe ngale komkhawulo.
Izitsha ze-Cryogenic zenziwa nge-polypropylene ewugqinsi ezivumela ukuthi zibambelele ngaphansi kwamazinga okushisa abandayo futhi ziphothwe ku-centrifuge ngesivinini esingaphezu kuka-25,000xg noma ngaphezulu.
Inkinga ilele kukepisi lokuvala elisetshenziselwa ukuvikela i-cryovial.
Ukuze i-cryovial ivikele kahle izicubu, iseli noma isampula yegciwane eliqukethe, ikepisi kufanele lijike ngokuphelele bese lenza isivalo esingavuzi.
Igebe elincane lizovumela ukuhwamuka kanye nokungcoliswa kwengozi.
Imizamo enzima yenziwa abakhiqizi be-cryovial ukuze bakhiqize uphawu lwekhwalithi ephezulu olungafaka i-silicon o-ring kanye/noma ukucushwa okuwugqinsi kokufihlwa ikepisi phansi ngokugcwele.
Leli izinga lalokho okungalethwa ngumkhiqizi we-cryovial.
Ekugcineni impumelelo noma ukwehluleka kwe-cryovial ukulondoloza isampula yokuwa kuchwepheshe zelabhu ukuze kuqinisekiswe ukuthi uphawu oluhle lwenziwe.
Uma uphawu luphansi, futhi ngisho nasezimeni lapho ikepisi livalwe kahle, i-nitrogen ewuketshezi ingangena ku-cryovial lapho icwiliswa ku-nitrogen yesigaba soketshezi.
Uma isampula incibilika ngokushesha okukhulu, i-nitrogen ewuketshezi izokhula ngokushesha futhi ibangele okuqukethwe okucindezelwe ukuthi kuqhume futhi kuthumele izingcezu zepulasitiki ezandleni nasebusweni banoma ubani oneshwa lokuba seduze.
Ngakho-ke, ngokuhlukile okungavamile, abakhiqizi be-cryovial badinga abasabalalisi babo ukuthi babonise ngesibindi umshwana wokuzihlangula ukuze bangasebenzisi ama-cryovials abo ngaphandle kwesigaba segesi se-nitrogen ewuketshezi (engaba ngu-180 kuya ku-186C).
Usengakhanyisa ngokushesha okuqukethwe okufriza ku-cryovial ngokuyicwilisa kancane esigabeni se-nitrogen esiwuketshezi;ziqinile ngokwanele futhi ngeke ukuqhekeka.
Ingabe ufuna ukufunda okwengeziwe ngezingozi zokugcina izitsha ze-cryogenic ku-liquid phase liquid nitrogen?
Nansi indatshana evela ku-UCLA's Center for Laboratory Safety ebhala ngokulimala ngenxa yokuqhuma kwe-cryovial.
Isikhathi sokuthumela: Apr-21-2022